Moehario Lucky H
Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
J Infect Dev Ctries. 2009 Sep 15;3(8):579-84. doi: 10.3855/jidc.548.
Typhoid fever remains a worldwide problem, but it is particularly common in Southeast Asia, including Indonesia. The causative agent Salmonella Typhi is known to have significant genome plasticity.
This study describes genetic fingerprints using restriction fragment length polymorphism with SpeI of chromosomal DNA and pulsed-field gel electrophoresis (PFGE) of S. Typhi isolated from different geographic areas spreading from west to east across Indonesia.
A total of 33 SpeI digested S. Typhi chromosomal DNA gave 22 schizotypes, 20 pulsotypes, and 12 subtypes indicating genomic diversity and the presence of more than one clone of S. Typhi. Cluster analysis at a degree of similarity of >or=80% showed four clusters, three of which were associated with geographic area. One cluster (Dice coefficient 0.727-1.000) contained isolates from three different geographic areas (Jakarta, Makasar, Jayapura), spread across Indonesia.
Genetic fingerprinting of S. Typhi in Indonesia showed the presence of endemic strains in localized geographic areas, as well as the movement of one strain type throughout the archipelago.
伤寒热仍是一个全球性问题，但在包括印度尼西亚在内的东南亚地区尤为常见。已知致病因子伤寒沙门氏菌具有显著的基因组可塑性。
本研究描述了利用染色体DNA的SpeI限制性片段长度多态性和从印度尼西亚西部到东部不同地理区域分离的伤寒沙门氏菌的脉冲场凝胶电泳（PFGE）进行基因指纹分析。
总共33个经SpeI消化的伤寒沙门氏菌染色体DNA产生了22种裂解型、20种脉冲型和12种亚型，表明基因组存在多样性以及存在不止一种伤寒沙门氏菌克隆。相似度≥80%的聚类分析显示有四个聚类，其中三个与地理区域相关。一个聚类（戴斯系数0.727 - 1.000）包含来自印度尼西亚不同三个地理区域（雅加达、望加锡、查亚普拉）的分离株。
印度尼西亚伤寒沙门氏菌的基因指纹分析表明，在局部地理区域存在地方菌株，以及一种菌株类型在整个群岛的传播。
