Wang Ning, Li Yongxian, Zheng Feiyun, Liu Chunfeng, Li Qi, Gu Guoxian
Jiangnan University, Key Laboratory of Industrial Biotechnology of Ministry of Education, Wuxi 214122, China.
Se Pu. 2009 May;27(3):372-5.
A method for the determination of xanthohumol in beer using solid-phase extraction-high performance liquid chromatography (SPE-HPLC) has been developed. A Waters Sep-Pak C18 column was used to extract and clean-up the sample. The separation was achieved on a reversed-phase Agilent Zorbax Eclipse XDB-C18 (250 mm x 4.6 mm, 5 microm) in a linear gradient, and the mobile phases were consisted of water (containing 0.1% formic acid) (A) and methanol (B) with a flow rate of 0.4 mL/min. In addition, the column temperature was maintained at 25 degrees C. The detection wavelength was set at 370 nm. There was a good linear relationship (r2 = 1) in the range of 0.5 - 500 microg/L. The average recoveries were between 91.21% and 95.58% with the relative standard deviations less than 2%. The limit of detection was 0.24 microg/L and the limit of quantification was 0.80 microg/L. It was proved to be a convenient and accurate method for the analysis of xanthohumol content in beer.
已开发出一种使用固相萃取-高效液相色谱法（SPE-HPLC）测定啤酒中黄腐酚的方法。采用沃特世Sep-Pak C18柱对样品进行萃取和净化。在反相安捷伦Zorbax Eclipse XDB-C18（250 mm×4.6 mm，5微米）柱上以线性梯度进行分离，流动相由水（含0.1%甲酸）（A）和甲醇（B）组成，流速为0.4 mL/min。此外，柱温保持在25℃。检测波长设定为370 nm。在0.5 - 500μg/L范围内线性关系良好（r2 = 1）。平均回收率在91.21%至95.58%之间，相对标准偏差小于2%。检测限为0.24μg/L，定量限为0.80μg/L。该方法被证明是一种分析啤酒中黄腐酚含量的简便、准确的方法。
