State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, PR China.
J Sep Sci. 2011 Jun;34(12):1463-8. doi: 10.1002/jssc.201000930. Epub 2011 May 5.
In this study, silica-coated magnetic nanoparticles (Fe(3)O(4)/SiO(2) NPs) modified by cetyltrimethylammonium bromide (CTAB) were synthesized. They were successfully applied for extraction of xanthohumol in beer based on magnetic mixed hemimicelles solid-phase extraction (MMHSPE) coupled with high-performance liquid chromatography-ultraviolet determination. The main factors influencing the extraction efficiency including the surfactant amount, the beer pH, the extraction time, the desorption condition and the maximum extraction beer volume were optimized. Under the optimized conditions, a concentration factor of 60 was achieved by extracting 120 mL beer sample using MMHSPE and the detection limit of xanthohumol is 0.0006 mg/L. The proposed method was successfully applied for determination of xanthohumol in various beer samples with the xanthohumol contents in the range of 0.031-0.567 mg/L. The satisfactory recoveries (90-103%) were obtained in analyzing spiked beer samples.
在这项研究中，合成了经十六烷基三甲基溴化铵（CTAB）修饰的硅涂层磁性纳米粒子（Fe3O4/SiO2 NPs）。它们成功地应用于基于磁性混合胶束固相萃取（MMHSPE）结合高效液相色谱-紫外检测的啤酒中黄腐酚的提取。优化了影响萃取效率的主要因素，包括表面活性剂用量、啤酒 pH 值、萃取时间、解吸条件和最大萃取啤酒体积。在优化条件下，通过使用 MMHSPE 从 120 mL 啤酒样品中提取，浓缩因子达到 60，黄腐酚的检测限为 0.0006 mg/L。该方法成功地应用于各种啤酒样品中黄腐酚的测定，黄腐酚含量在 0.031-0.567 mg/L 范围内。在分析加标啤酒样品时，获得了令人满意的回收率（90-103%）。
