Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
J Biosci Bioeng. 2009 Nov;108(5):446-9. doi: 10.1016/j.jbiosc.2009.05.005.
We established a novel method, insertional chromatin immunoprecipitation (iChIP), for isolation of specific genomic regions. In iChIP, specific genomic domains are immunoprecipitated with antibody against a tag, which is fused to the DNA-binding domain of an exogenous DNA-binding protein, whose recognition sequence is inserted into the genomic domains of interest. The iChIP method will be a useful tool for dissecting chromatin structure of genomic region of interest.
我们建立了一种新的方法，插入染色质免疫沉淀（iChIP），用于分离特定的基因组区域。在 iChIP 中，通过针对融合到外源性 DNA 结合蛋白 DNA 结合域的标签的抗体来免疫沉淀特定的基因组结构域，该外源性 DNA 结合蛋白的识别序列插入到感兴趣的基因组区域中。iChIP 方法将成为研究感兴趣的基因组区域染色质结构的有用工具。
