Gupta Nidhi, Singh Sunita, Marwah Nisha, Kumar Sanjay, Chabra Sonia, Sen Rajeev
Department of Pathology, Post Graduate Institute of Medical Sciences, Rohtak-124 001, Haryana, India.
Indian J Pathol Microbiol. 2009 Oct-Dec;52(4):482-5. doi: 10.4103/0377-4929.56127.
This study was conducted to evaluate the expression of HER-2/neu oncogene in the lesions of the uterine cervix and to determine its correlation with histological type of malignancy, grade and clinical stage of presentation. One hundred cervical specimens were included in this study. These comprised cases with diagnosis of benign epithelial lesions, squamous cell carcinoma, adenocarcinoma, carcinoma cervix with glandular differentiation and cervical intraepithelial neoplasia. HER-2/neu immunostaining was performed by streptovidin-biotin peroxidase method. Higher expression of HER-2/neu was noted in malignant lesions as compared to benign lesions. Intensity of staining also correlated with clinical stage of presentation, lymph node metastasis and presence of parametrial extension. The over-expression of HER-2 oncoprotein is associated with poor prognosis, metastatic potential and aggressive biological behavior.
本研究旨在评估HER-2/neu癌基因在子宫颈病变中的表达，并确定其与恶性肿瘤组织学类型、分级及临床分期的相关性。本研究纳入了100例宫颈标本。这些标本包括诊断为良性上皮病变、鳞状细胞癌、腺癌、伴有腺分化的子宫颈癌及宫颈上皮内瘤变的病例。采用链霉亲和素-生物素过氧化物酶法进行HER-2/neu免疫染色。与良性病变相比，恶性病变中HER-2/neu表达更高。染色强度也与临床分期、淋巴结转移及宫旁组织受累情况相关。HER-2癌蛋白的过表达与预后不良、转移潜能及侵袭性生物学行为有关。
