Rahmani Arshad H, Babiker Ali Yousif, Alsahli Mohammed A, Almatroodi Saleh A, Husain Nazik Elmalaika O S
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi Arabia.
Department of Histopathology and Cytology, College of Medical Laboratories Science, University of Sciences and Technology, Omdurman, Sudan.
Open Access Maced J Med Sci. 2018 Feb 10;6(2):263-268. doi: 10.3889/oamjms.2018.089. eCollection 2018 Feb 15.
Angiogenesis plays a pivotal role in the progression of tumours through the formation of new blood vessels. Vascular endothelial growth factor (VEGF) is a chief factor responsible for inducing and regulating angiogenesis. Additionally, the human epidermal growth factor receptor family of receptors also plays an important role in the pathogenesis of tumours.
This study aimed to examine the association between VEGF and Her-2 protein expression and its correlation with clinic-pathological characteristics; in particular, prognosis.
A total of 65 cases of cervical carcinoma and 10 samples of inflammatory lesions were evaluated for VEGF and Her-2 protein expression.
Expression of VEGF and Her-2 was detected in 63.07% and 43.07% in cervical carcinoma cases respectively whereas control cases did not show any expression. The difference in the expression pattern of both markers comparing cancer and control cases was statistically significant (p < 0.05). However, no significant difference in the expression pattern of VEGF protein was observed among the different grades and stages of tumours (p > 0.05). Comparing different grades of a tumour, expression of Her-2 was detected in 31.8% of well-differentiated tumours, 36.0 % in moderately differentiated tumours and 66.66 % in poorly differentiated cancers. The expression of Her-2 was increased in high-grade tumours, and the difference of expression level between tumour grades was statistically significant (p < 0.05). The expression level of Her-2 protein was not correlated with the stage of a tumour (p > 0.05).
The present study supports earlier findings that over-expression / up-regulation of VEGF and Her - 2 is linked with poor prognosis and may play a vital role in the development and progression of cervical cancer.
血管生成通过形成新血管在肿瘤进展中起关键作用。血管内皮生长因子（VEGF）是诱导和调节血管生成的主要因子。此外，人表皮生长因子受体家族受体在肿瘤发病机制中也起重要作用。
本研究旨在探讨VEGF与Her-2蛋白表达之间的关联及其与临床病理特征（尤其是预后）的相关性。
对65例宫颈癌病例和10例炎性病变样本进行VEGF和Her-2蛋白表达评估。
宫颈癌病例中VEGF和Her-2的表达分别检测到63.07%和43.07%，而对照病例未显示任何表达。比较癌症病例和对照病例，两种标志物表达模式的差异具有统计学意义（p < 0.05）。然而，在肿瘤的不同分级和分期中，未观察到VEGF蛋白表达模式的显著差异（p > 0.05）。比较不同分级的肿瘤，Her-2在31.8%的高分化肿瘤、36.0%的中分化肿瘤和66.66% 的低分化癌中表达。Her-2在高级别肿瘤中的表达增加，肿瘤分级之间的表达水平差异具有统计学意义（p < 0.05）。Her-2蛋白的表达水平与肿瘤分期无关（p > 0.05）。
本研究支持早期研究结果，即VEGF和Her - 2的过表达/上调与预后不良相关，可能在宫颈癌的发生和发展中起重要作用。
