Schmidt D, Roznoski M, Ebert M H
Department of Psychiatry, Vanderbilt University School of Medicine Nashville, TN 37232.
Biomed Chromatogr. 1990 Sep;4(5):215-20. doi: 10.1002/bmc.1130040509.
The application of reductive coulometric electrochemical detection for analysis of the monoamine neurotransmitters norepinephrine, dopamine, and serotonin and their common metabolites in brain and cerebrospinal fluid following separation by isocratic high performance liquid chromatography is described. The high sensitivity and screening capabilities of coulometric electrodes permits the accurate quantitation of as little as 3-5 pg of these compounds in tissue following a simple single step purification procedure. Moreover, comparison of peak height ratios obtained from analysis of authentic reference standards and tissue samples at selected multiple electrode potentials provides a straightforward means for qualitative evaluation of peak identification and purity during analysis of biological samples. The method is comparatively inexpensive and precise within and between day coefficients of variation for most compounds range from 2-5%. Thirty samples can be run in duplicate in a 24 h period.
描述了在等度高效液相色谱分离后，采用还原库仑电化学检测法分析大脑和脑脊液中的单胺类神经递质去甲肾上腺素、多巴胺和5-羟色胺及其常见代谢产物。库仑电极的高灵敏度和筛选能力使得在经过简单的单步纯化程序后，能够准确地定量检测组织中低至3-5皮克的这些化合物。此外，在选定的多个电极电位下，对真实参考标准品和组织样品分析得到的峰高比进行比较，为生物样品分析过程中的峰识别和纯度定性评估提供了一种直接的方法。该方法成本相对较低，大多数化合物在日内和日间变异系数范围内的精密度为2%-5%。在24小时内可以对30个样品进行一式两份的检测。
