[小干扰RNA沉默Dicer对人胶质瘤细胞生物学特性的影响]
[The effect of silencing Dicer by small interference RNA on the biological characteristics of human glioma cells].
作者信息
Zhang An-ling, Kang Chun-sheng, Han Lei, Wang Guang-xiu, Jia Zhi-fan, Pu Pei-yu
机构信息
Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-oncology, Tianjin Neurological Institute, Tianjin, 300052, PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Oct;26(5):521-4. doi: 10.3760/cma.j.issn.1003-9406.2009.05.009.
OBJECTIVE
To study the effect of silencing Dicer by small interference RNA (siRNA) to suppress the global microRNA (miRNAs) expression on the biological characteristics of TJ905 glioblastoma cells.
METHODS
The silencing effect of RNA interference on Dicer expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis and immunofluorescence staining. The cell proliferation rate and cell cycle kinetics were detected by MTT assay and flow cytometry respectively, and the cell invasive ability was evaluated by transwell assay.
RESULTS
The siRNA targeting Dicer suppressed the expression of Dicer in TJ905 cells. Meanwhile, the proliferation activity and invasive ability were significantly enhanced in cells transfected with Dicer siRNA compared to those cells transfected with scrambled siRNA and the control cells.
CONCLUSION
Suppression of Dicer expression renders the glioma cells harboring more aggressive phenotype. This preliminary finding suggests that global lower expression of miRNAs may play an oncogenic role.
目的
研究通过小干扰RNA（siRNA）沉默Dicer以抑制整体微小RNA（miRNA）表达对TJ905胶质母细胞瘤细胞生物学特性的影响。
方法
通过逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹分析和免疫荧光染色评估RNA干扰对Dicer表达的沉默效果。分别采用MTT法和流式细胞术检测细胞增殖率和细胞周期动力学，通过Transwell实验评估细胞侵袭能力。
结果
靶向Dicer的siRNA抑制了TJ905细胞中Dicer的表达。同时，与转染乱序siRNA的细胞和对照细胞相比，转染Dicer siRNA的细胞增殖活性和侵袭能力显著增强。
结论
抑制Dicer表达使胶质瘤细胞具有更具侵袭性的表型。这一初步发现表明，整体较低的miRNA表达可能发挥致癌作用。
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