Department of Life and Environmental Sciences, Chiba Institute of Technology, Narashino, Chiba, Japan.
FEBS Lett. 2010 Oct 22;584(20):4313-8. doi: 10.1016/j.febslet.2010.09.031. Epub 2010 Sep 24.
Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA.
双链 RNA 通过一种称为 RNA 干扰 (RNAi) 或转录后基因沉默的进化保守过程来抑制同源基因的表达。双结构域核酸酶 Dicer 被认为是负责产生短干扰 RNA (siRNA) 的蛋白质。在我们的实验中，Rex 的过表达降低了短发夹 RNA (shRNA)介导的 RNAi 的效率。Dicer 与 Rex 的相互作用抑制了 shRNA 向 siRNA 的转化。这些结果表明，Dicer 与 HTLV-I Rex 的相互作用抑制了 Dicer 的活性，从而降低了 shRNA 向 siRNA 转化的效率。
