Yan Wei, Qian Chun-fa, Shi Lei, Qian Jin, Fu Zhen, Liu Ning, Lu Ai-lin, You Yong-ping
Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Oct;26(5):525-8. doi: 10.3760/cma.j.issn.1003-9406.2009.05.010.
To investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells.
The invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively.
Osteopontin small interference RNA (siRNA) inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated.
Osteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl 2 level. It suggests that osteopontin plays an important role in human glioma progression.
探讨骨桥蛋白沉默对U251细胞侵袭和凋亡的影响。
分别采用侵袭实验、流式细胞术、蛋白质印迹法及实时荧光定量PCR法检测细胞侵袭、凋亡及尿激酶型纤溶酶原激活物（uPA）、基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）水平。
骨桥蛋白小干扰RNA（siRNA）抑制U251细胞中骨桥蛋白表达及细胞侵袭，促进细胞凋亡。此外，Bcl-2、uPA、MMP-2和MMP-9表达降低，而Bax水平升高。
骨桥蛋白siRNA可通过下调uPA、MMP-2和MMP-9水平抑制U251细胞侵袭，并通过诱导Bax表达和抑制Bcl-2水平促进细胞凋亡。提示骨桥蛋白在人类胶质瘤进展中起重要作用。
