Sun Yanan, Liu Ming, Yang Baofeng, Lu Jianguang, Li Baoxin
The Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Cancer Invest. 2008 Dec;26(10):984-9. doi: 10.1080/07357900802072897.
The purpose of this study was to explore the inhibiting role of MMP-9 gene silence in the invasive ability and growth of laryngeal squamous cell carcinoma (LSCC) by lentivirus mediated RNA interference. MMP-9-RNAi-lentivirus and the control lentivirus (GFP-lentivirus) were transfected into Hep-2 cells. Gelatin zymography showed the proteins expression of MMP-9 were knockdown in the MMP-9 siRNA transfected Hep-2 cells. The invasive activity and viability of MMP-9 siRNA treated Hep-2 cells were decreased than the control cells measured with modified Boyden chamber assay and MTT assay. In animal experiment, 20 nude mice bearing Hep-2 cell tumor were randomly separated into the experimental and the control groups. The former were intratumorally injected with MMP-9-RNAi-lentivirus, and the later were injected with equivalent dose of GFP-lentivirus. Results showed the average weight and volume of tumor in MMP-9-RNAi-lentivirus treated group were significantly lower than those in the control group (P < .01). The protein expressions of MMP-9 were downregulated in tumors of MMP-9-RNAi-lentivirus treatment. The PCNA index was obviously lower in the tumors of treated group than that in the control group (P < .01). These results suggest that MMP-9 gene silence by lentivirus mediated RNA interference can inhibit invasion and growth of LSCC.
本研究旨在通过慢病毒介导的RNA干扰探索MMP-9基因沉默对喉鳞状细胞癌（LSCC）侵袭能力和生长的抑制作用。将MMP-9-RNAi-慢病毒和对照慢病毒（GFP-慢病毒）转染至Hep-2细胞。明胶酶谱分析显示，在转染MMP-9 siRNA的Hep-2细胞中，MMP-9的蛋白表达被敲低。与用改良Boyden小室试验和MTT试验检测的对照细胞相比，MMP-9 siRNA处理的Hep-2细胞的侵袭活性和活力降低。在动物实验中，将20只荷Hep-2细胞瘤的裸鼠随机分为实验组和对照组。前者瘤内注射MMP-9-RNAi-慢病毒，后者注射等量的GFP-慢病毒。结果显示，MMP-9-RNAi-慢病毒处理组的肿瘤平均重量和体积显著低于对照组（P <.01）。MMP-9-RNAi-慢病毒处理的肿瘤中MMP-9的蛋白表达下调。处理组肿瘤的PCNA指数明显低于对照组（P <.01）。这些结果表明，慢病毒介导的RNA干扰使MMP-9基因沉默可抑制LSCC的侵袭和生长。
