Thiele J, Timmer J, Jansen B, Zankovich R, Fischer R
Institute of Pathology, University of Cologne, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(3):125-31. doi: 10.1007/BF02899396.
In seven patients with chronic myeloid leukemia (CML) and ultrastructural and morphometric study was performed on neutrophilic granulopoiesis in bone marrow trephine biopsies. Bone marrow specimens from five patients without hematological abnormalities served as controls. In stable phases of CML, abnormalities of the maturing granulocytic lineage were most conspicuously expressed by an infrequently occurring nuclear disfiguration (blebs and disturbed bridging of segments). Morphometric evaluation included the numbers of azurphil (primary) and specific (secondary) granules, the cisternal length of the endoplasmic reticulum and the area of the mitochondrial profiles. These variables could be determined in early and late myeloblasts, promyelocytes, metamyelocytes, band cells and mature polymorphonuclear granulocytes. Statistical analysis with regard to control specimens demonstrated no significant differences in the total amount of neutrophil granules or of the other cell organelles.
对7例慢性髓性白血病（CML）患者进行了骨髓环钻活检中性粒细胞生成的超微结构和形态计量学研究。选取5例无血液学异常患者的骨髓标本作为对照。在CML的稳定期，成熟粒细胞系的异常最明显地表现为核形态异常（泡状和节段间桥接紊乱），这种情况很少发生。形态计量学评估包括嗜天青（初级）和特异性（次级）颗粒的数量、内质网池长度和线粒体轮廓面积。这些变量可以在早幼粒细胞、中幼粒细胞、晚幼粒细胞、杆状核细胞和成熟多形核粒细胞中测定。与对照标本的统计分析表明，中性粒细胞颗粒或其他细胞器的总量无显著差异。
