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结核分枝杆菌复苏促进因子相互作用蛋白RipA的表达、纯化、结晶及初步X射线晶体学分析

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the resuscitation promoting factor interacting protein RipA from M. tuberculosis.

作者信息

Ruggiero Alessia, Squeglia Flavia, Esposito Carla, Marasco Daniela, Pedone Emilia, Pedone Carlo, Berisio Rita

机构信息

Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16. I-80134 Napoli, Italy.

出版信息

Protein Pept Lett. 2010 Jan;17(1):70-3. doi: 10.2174/092986610789909557.

DOI:10.2174/092986610789909557
PMID:19807672
Abstract

RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and an abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.

摘要

RipA是结核分枝杆菌的一种重要生长因子。其缺失会导致细菌生长减缓以及异常表型。包含预测催化结构域的RipA C末端片段（263 - 472）已通过气相扩散法成功结晶。该结构已通过多波长反常色散解析，原子分辨率精修正在进行中。

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