Rv0100，一种在结核分枝杆菌中被提议的酰基载体蛋白：表达、纯化及结晶
Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization.
作者信息
Bondoc Jasper Marc G, Gutka Hiten J, Almutairi Mashal M, Patwell Ryan, Rutter Maxwell W, Wolf Nina M, Samudrala Ram, Mehboob Shahila, Movahedzadeh Farahnaz
机构信息
Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Department of Biomedical Informatics, Jacobs School of Medicine and Biomedical Sciences, State University of New York (SUNY), University at Buffalo, 77 Goodell Street, Buffalo, NY 14203, USA.
出版信息
Acta Crystallogr F Struct Biol Commun. 2019 Oct 1;75(Pt 10):646-651. doi: 10.1107/S2053230X19012652. Epub 2019 Sep 24.
Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.
酰基载体蛋白（ACPs）是原核生物脂肪酸生物合成中的重要组成部分。Rv0100被预测为结核分枝杆菌（结核病的病原体）中的一种必需酰基载体蛋白，因此有潜力成为新型抗结核药物靶点。在此，报告了以耻垢分枝杆菌为宿主成功克隆和纯化Rv0100的过程。获得了纯化蛋白的晶体，其衍射分辨率达到1.9 Å。总体而言，这项工作为未来针对这个潜在的新型药物靶点进行药物发现和开发奠定了基础。
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