Department of Hand Surgery, Huashan Hospital, Fudan University Shanghai 200040, China.
Neurosci Res. 2010 Jan;66(1):46-52. doi: 10.1016/j.neures.2009.09.1711. Epub 2009 Oct 4.
Bone marrow stromal stem cells (MSCs), which normally differentiate into mesenchymal derivatives, have recently reported to trans-differentiate into neurons. However, the findings from different groups and interpretations have been challenged. The purpose of this paper is to re-evaluate the phenomenon of neuronal trans-differentiation of MSCs and compare the expression levels of neurotrophins in rMSCs and neuronal-like phenotypes derived from rMSCs. We put rMSCs in 2-mercaptoethanol and 2% dimethylsulfoxide for 5h. Then, the cells were transferred to neuronal induction media composed of DMEM+10%FBS, 10ug/L basic fibroblast growth factor, 10ug/L human epidermal growth factor, 1mmol dibutyryl cyclicn AMP and 0.5mmol isobutylmethylxanthine for 7 days and 14 days. The study demonstrated that the level of BDNF, NGF, NT3, CNTF and GDNF of rMSCs is remarkably higher in rMSCs than the neuronal-like phenotypes, especially CNTF. The expression level of these neurotrophins did not change significantly after enduring induction. We believed that rMSCs can trans-differentiate into neuronal-like phenotype under certain conditions. The non-induced rMSCs has a dynamic expression profile of neurotrophins and may serves as a better tool than the trans-differentiated rMSCs for transplant therapy.
骨髓基质干细胞（MSCs）通常分化为间充质衍生物，最近有报道称其可转分化为神经元。然而，不同研究组的发现和解释受到了挑战。本文旨在重新评估 MSCs 神经元转分化的现象，并比较 rMSCs 中神经生长因子的表达水平和 rMSCs 来源的神经元样表型。我们将 rMSCs 置于 2-巯基乙醇和 2%二甲基亚砜中 5 小时。然后，将细胞转移到含有 DMEM+10%FBS、10ug/L 碱性成纤维细胞生长因子、10ug/L 人表皮生长因子、1mmol 二丁酰环 AMP 和 0.5mmol 异丁基甲基黄嘌呤的神经元诱导培养基中诱导 7 天和 14 天。研究表明，rMSCs 中的 BDNF、NGF、NT3、CNTF 和 GDNF 水平明显高于神经元样表型，尤其是 CNTF。在诱导后，这些神经生长因子的表达水平没有显著变化。我们认为，rMSCs 在某些条件下可以转分化为神经元样表型。未诱导的 rMSCs 具有神经生长因子的动态表达谱，可能比转分化的 rMSCs 更适合移植治疗。
