Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, Korea.
J Microbiol Biotechnol. 2009 Sep;19(9):881-7. doi: 10.4014/jmb.0902.090.
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78%of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5%of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.
从海洋鱼类的肠道中分离到 44 株二十碳五烯酸（EPA）产生菌。其中一株 EPA 产量最高（占总脂肪酸的 4.78%）的菌株，根据其 16S rRNA 序列被鉴定为希瓦氏菌属 BR-2。EPA 含量在细胞生长的指数中期达到最高水平，随着细胞的进一步生长逐渐降低。从希瓦氏菌属 BR-2 的基因组 DNA 构建的 cosmid 文库中分离到一个包含 pfaA-E 的 EPA 生物合成基因簇的 cosmid DNA，命名为 pCosEPA-BR2。含有 pCosEPA-BR2 的大肠杆菌克隆产生的 EPA 最高达到总脂肪酸的 7.5%，证实了克隆基因簇的 EPA 生物合成活性。
