Hirota Kikue, Nodasaka Yoshinobu, Orikasa Yoshitake, Okuyama Hidetoshi, Yumoto Isao
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2355-2359. doi: 10.1099/ijs.0.63804-0.
An eicosapentaenoic acid-producing bacterium, previously described as Shewanella sp. strain SCRC-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16S rRNA gene sequence analysis and DNA-DNA hybridization. The isolate was Gram-negative, rod-shaped and motile by using polar flagella. The strain grew at 4-32 degrees C; the optimum growth temperature was 27 degrees C. NaCl was required for growth. The major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its DNA G+C content was 42.8 mol%. The whole-cell fatty acids mainly (above 5 %) consisted of iso-C(13 : 0), iso-C(15 : 0), C(16 : 0), C(16 : 1)omega7c, C(18 : 1)omega7c and C(20 : 5)omega3 (eicosapentaenoic acid). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCRC-2738T was related closely (sequence similarities above 99 %) to Shewanella marinintestina (99.3 %), Shewanella sairae (99.3 %) and Shewanella schlegeliana (99.2 %). DNA-DNA hybridization and phenotypic characteristics confirmed that strain SCRC-2738T merited classification as a novel species of the genus Shewanella, for which the name Shewanella pneumatophori sp. nov. is proposed. The type strain is SCRC-2738T (=JCM 13187T=NCIMB 14060T).
一株产二十碳五烯酸的细菌，先前被描述为希瓦氏菌属菌株SCRC-2738，通过表型特征、化学分类分析、16S rRNA基因序列分析和DNA-DNA杂交进行分类。该分离株为革兰氏阴性、杆状，通过极生鞭毛运动。该菌株在4-32℃生长；最适生长温度为27℃。生长需要氯化钠。主要类异戊二烯醌为泛醌-7和泛醌-8，其DNA G+C含量为42.8 mol%。全细胞脂肪酸主要（超过5%）由异-C(13 : 0)、异-C(15 : 0)、C(16 : 0)、C(16 : 1)ω7c、C(18 : 1)ω7c和C(20 : 5)ω3（二十碳五烯酸）组成。基于16S rRNA基因序列的系统发育分析表明，菌株SCRC-2738T与海洋希瓦氏菌（99.3%）、赛拉希瓦氏菌（99.3%）和施氏希瓦氏菌（99.2%）密切相关（序列相似性超过99%）。DNA-DNA杂交和表型特征证实，菌株SCRC-2738T应分类为希瓦氏菌属的一个新物种，为此提出新名称希瓦氏气单胞菌。模式菌株为SCRC-2738T（=JCM 13187T=NCIMB 14060T）。
