Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Rm 220, No. 2 Biotech. Ave, 12 Miles, Tai Po Road, Shatin, N.T., Hong Kong, China.
Phytochem Anal. 2010 Mar-Apr;21(2):137-43. doi: 10.1002/pca.1168.
Aconites, with aconite alkaloids as the major therapeutic and toxic components, are used for the treatment of analgesic, antirheumatic and neurological symptoms. Quantification of the aconite alkaloids is important for the quality control of aconite-containing drugs.
To establish a validated capillary zone electrophoresis (CZE) method for the simultaneous determination of six major alkaloids, namely aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine, in crude and processed aconite roots.
The CZE method was optimised and validated using a stability-indicating method. The optimised running buffer was a mixture of 200 mm Tris, 150 mm perchloric acid and 40% 1,4-dioxane (pH 7.8) with the capillary thermostated at 25 degrees C.
Using the optimised method, six aconite alkaloids were well separated. The established method showed good precision, accuracy and recovery. Contents of these alkaloids in crude and processed aconites were determined and it was observed that the levels of individual alkaloids varied between samples.
The developed CZE method was reliable for the quality control of aconites contained in herbal medicines. The method could also be used as an approach for toxicological studies.
乌头类植物以乌头生物碱为主要治疗和毒性成分，用于治疗止痛、抗风湿和神经症状。乌头生物碱的定量分析对于含乌头类药物的质量控制非常重要。
建立一种经验证的毛细管区带电泳（CZE）方法，用于同时测定生乌头和炮制乌头根中 6 种主要生物碱，即乌头碱、次乌头碱、新乌头碱、苯甲酰乌头碱、苯甲酰次乌头碱和苯甲酰新乌头碱。
采用稳定性指示方法对 CZE 方法进行优化和验证。优化的运行缓冲液为 200mm Tris、150mm 高氯酸和 40%1,4-二恶烷（pH7.8）的混合物，毛细管在 25°C 下恒温。
使用优化的方法，6 种乌头生物碱得到了很好的分离。所建立的方法具有良好的精密度、准确性和回收率。测定了生乌头和炮制乌头中这些生物碱的含量，观察到各生物碱的水平在样品之间有所不同。
所开发的 CZE 方法可用于草药中乌头类药物的质量控制。该方法也可用于毒理学研究。
