Powell Katie, Low Pauline, McDonnell P Ann, Laakso E-Liisa, Ralph Stephen J
School of Medical Science, Griffith University, Gold Coast, Queensland, Australia.
Photomed Laser Surg. 2010 Feb;28(1):115-23. doi: 10.1089/pho.2008.2445.
This study compared the effects of different doses (J/cm(2)) of laser phototherapy at wavelengths of either 780, 830, or 904 nm on human breast carcinoma, melanoma, and immortalized human mammary epithelial cell lines in vitro. In addition, we examined whether laser irradiation would malignantly transform the murine fibroblast NIH3T3 cell line.
Laser phototherapy is used in the clinical treatment of breast cancer-related lymphoedema, despite limited safety information. This study contributes to systematically developing guidelines for the safe use of laser in breast cancer-related lymphoedema.
Human breast adenocarcinoma (MCF-7), human breast ductal carcinoma with melanomic genotypic traits (MDA-MB-435S), and immortalized human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of laser. MCF-7 cells were further irradiated with two and three exposures of each laser wavelength. Cell proliferation was assessed 24 h after irradiation.
Although certain doses of laser increased MCF-7 cell proliferation, multiple exposures had either no effect or showed negative dose response relationships. No sign of malignant transformation of cells by laser phototherapy was detected under the conditions applied here.
Before a definitive conclusion can be made regarding the safety of laser for breast cancer-related lymphoedema, further in vivo research is required.
本研究比较了波长为780、830或904nm的不同剂量（J/cm²）激光光疗对人乳腺癌、黑色素瘤和永生化人乳腺上皮细胞系的体外影响。此外，我们还研究了激光照射是否会使小鼠成纤维细胞NIH3T3细胞系发生恶性转化。
尽管安全信息有限，但激光光疗仍用于乳腺癌相关淋巴水肿的临床治疗。本研究有助于系统地制定激光在乳腺癌相关淋巴水肿中安全使用的指南。
对人乳腺腺癌（MCF-7）、具有黑色素瘤基因型特征的人乳腺导管癌（MDA-MB-435S）和永生化人乳腺上皮（SVCT和Bre80hTERT）细胞系进行单次激光照射。对MCF-7细胞进一步进行每种激光波长的两次和三次照射。照射后24小时评估细胞增殖情况。
虽然某些剂量的激光可增加MCF-7细胞增殖，但多次照射要么没有效果，要么呈现负剂量反应关系。在本研究采用的条件下，未检测到激光光疗使细胞发生恶性转化的迹象。
在就激光用于乳腺癌相关淋巴水肿的安全性得出明确结论之前，需要进一步开展体内研究。
