Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305-5178, USA.
Am J Reprod Immunol. 2009 Nov;62(5):339-47. doi: 10.1111/j.1600-0897.2009.00743.x.
The rate of pre-term birth in the United States continues to rise despite several interventions. Induction of pro-inflammatory cytokines and chemokines has been implicated in the activation of the cascade of events resulting in pre-term labor. To date, no comprehensive panel of the cytokine profile in PTL has been published.
To address cytokine profiles in pre-term labor, levels of 19 plasma and amniotic fluid cytokines were measured using a multiplex immunoassay in an inflammation-induced murine model of pre-term labor.
Pro-inflammatory mediators, RANTES, KC, IL-6, and IL-12p40 were increased by 3 hr and remained high at 15 hr. Concentrations of KC, IL-6, IL-1beta, and MIP-1alpha were increased in the amniotic fluid at 15 hr. Plasma levels of anti-inflammatory mediators IL-10 and IL-13 at 15 hr were unchanged and decreased respectively.
These results suggest that stimulation of several pro-inflammatory cytokines occurs very early in the cascade of events and remains increased, whereas anti-inflammatory cytokines are either unchanged or decreased until the onset of delivery in an inflammation-induced mouse model of pre-term labor.
尽管采取了多种干预措施，美国的早产率仍持续上升。促炎细胞因子和趋化因子的诱导已被牵涉到导致早产的级联事件的激活中。迄今为止，尚未发表关于 PTL 中细胞因子谱的综合报告。
为了研究早产中的细胞因子谱，我们使用多重免疫分析法在早产的炎症诱导的小鼠模型中测量了 19 种血浆和羊水细胞因子的水平。
促炎介质 RANTES、KC、IL-6 和 IL-12p40 在 3 小时增加，并在 15 小时保持高水平。在 15 小时时，KC、IL-6、IL-1β和 MIP-1α的浓度在羊水中增加。在 15 小时时，抗炎介质 IL-10 和 IL-13 的血浆水平不变，分别减少。
这些结果表明，在级联事件中，几种促炎细胞因子的刺激很早就发生了，并且一直增加，而抗炎细胞因子在炎症诱导的早产小鼠模型中，要么不变，要么减少，直到分娩开始。
