Téllez Castillo C J, Montava Vilaplana R, Fernández Jiménez M, Ribes Fernández J M, Buesa Gómez J
Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia, Valencia, España.
An Pediatr (Barc). 2010 Jan;72(1):49-54. doi: 10.1016/j.anpedi.2009.08.002. Epub 2009 Oct 7.
Rotavirus is the leading cause of acute gastroenteritis in young children worldwide. Effective vaccines to prevent rotavirus infections are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter seasons.
To characterise G (VP7) and P (VP4) genotypes of rotaviruses causing acute gastroenteritis in children and to determine the prevalence of genotype G9 rotavirus in three public health areas in the provinces of Valencia and Castellon.
Five-hundred and forty-one stool samples were prospectively collected from infants and children with gastroenteritis in the period between October 2005 and September 2008. They were analysed for rotavirus by ELISA or by immunochromatography. G and P genotyping was performed by reverse transcription and PCR (RT-PCR).
G and P rotavirus genotypes were characterised in a total of 525 faecal samples (97%), resulting in a global predominance of strains G9P[8] (56.5%) and G1P[8] (29.9%). During the period of time studied, G9P[8] was the G/P combination most frequently detected during the rotavirus seasons 2005-2006 and 2006-2007, being present in 81.2% and 64.7% of the patients, respectively. However, during the 2007-2008 season, G1P[8] strains were the most frequently found (68.8%), with a sharp decrease in G9P[8] strains to 7.2% of the samples.
Rotavirus G9P[8] have spread rapidly and widely during the 2005-2006 and 2006-2007 seasons, replacing other previously dominant genotypes (G1, G4) in our geographic area. Its incidence has declined sharply in 2007-2008, in which G1P[8] was again the predominating genotype.
轮状病毒是全球幼儿急性胃肠炎的主要病因。目前已有有效的疫苗来预防轮状病毒感染，尽管其临床应用仍然有限，且轮状病毒仍会引发许多婴幼儿胃肠炎病例，主要集中在冬季。
鉴定导致儿童急性胃肠炎的轮状病毒的G（VP7）和P（VP4）基因型，并确定瓦伦西亚省和卡斯特利翁省三个公共卫生区域中G9基因型轮状病毒的流行情况。
在2005年10月至2008年9月期间，前瞻性收集了541份患胃肠炎婴幼儿和儿童的粪便样本。通过酶联免疫吸附测定（ELISA）或免疫层析法对样本进行轮状病毒分析。通过逆转录和聚合酶链反应（RT-PCR）进行G和P基因分型。
共对525份粪便样本（97%）进行了G和P轮状病毒基因型鉴定，结果显示G9P[8]株（56.5%）和G1P[8]株（29.9%）在总体上占主导地位。在研究期间，G9P[8]是2005 - 2006年和2006 - 2007年轮状病毒流行季节中最常检测到的G/P组合，分别占患者的81.2%和64.7%。然而，在2007 - 2008年流行季节，G1P[8]株最为常见（68.8%），G9P[8]株急剧下降至样本的7.2%。
轮状病毒G9P[8]在2005 - 2006年和2006 - 2007年期间迅速广泛传播，取代了我们所在地理区域先前的其他优势基因型（G1、G4）。其发病率在2007 - 2008年急剧下降，其中G1P[8]再次成为主导基因型。
