Grassi T, De Donno A, Guido M, Gabutti G
Department of Biological and Environmental Sciences and Technologies, Lab of Hygiene, University of Lecce, Italy.
J Prev Med Hyg. 2006 Dec;47(4):138-41.
Rotaviruses are the most common agents of diarrhoeal illness in infants and young children. Gastroenteritis caused by rotaviruses is also more likely to be associated with severe dehydration compared to other viral gastroenteritis. We determined the G-genotype of rotaviruses circulating in the Salento. During 2004, 144 stool samples were collected from subjects with a positive screening test and stored at -20 degrees C until confirmation could take place using molecular biology techniques. A reverse transcription-polymerase chain reaction (RT-PCR) assay for the amplification of gene VP7 of rotavirus was developed and used; G-genotype was determined by sequencing and phylogenetic analysis. A total of 101 stool samples were detected positive by RT-PCR and 35 were sequence analyzed and classified into G1, G2, G4, G9 and G12 type. The overall relative incidence of G-types in Salento is different to that of other developed countries. The most prevalent genotype was G2 while genotype G1 was present at low levels. It is also interesting to note the presence of G9 rotavirus, which is now recognized as the fifth globally important rotavirus genotype. In this study, therefore, we demonstrate the usefulness of a simple method for correctly determining the G genotypes circulating in a geographic region.
轮状病毒是婴幼儿腹泻疾病最常见的病原体。与其他病毒性肠胃炎相比，由轮状病毒引起的肠胃炎也更有可能与严重脱水相关。我们确定了在萨伦托地区传播的轮状病毒的G基因型。2004年期间，从筛查试验呈阳性的受试者中收集了144份粪便样本，并储存在-20摄氏度下，直到可以使用分子生物学技术进行确认。开发并使用了一种用于扩增轮状病毒基因VP7的逆转录-聚合酶链反应（RT-PCR）检测方法；通过测序和系统发育分析确定G基因型。共有101份粪便样本通过RT-PCR检测呈阳性，对其中35份进行了序列分析，并分类为G1、G2、G4、G9和G12型。萨伦托地区G型的总体相对发病率与其他发达国家不同。最常见的基因型是G2，而G1基因型的含量较低。同样值得注意的是G9轮状病毒的存在，它现在被认为是全球第五种重要的轮状病毒基因型。因此，在本研究中，我们证明了一种简单方法对于正确确定在一个地理区域传播的G基因型的有用性。
