基于 16S rRNA 基因的 PCR 技术检测和定量分析 Dehalogenimonas 和 "Dehalococcoides" 种群。
Detection and quantification of Dehalogenimonas and "Dehalococcoides" populations via PCR-based protocols targeting 16S rRNA genes.
机构信息
Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
出版信息
Appl Environ Microbiol. 2009 Dec;75(23):7560-4. doi: 10.1128/AEM.01938-09. Epub 2009 Oct 9.
Abstract
Members of the haloalkane dechlorinating genus Dehalogenimonas are distantly related to "Dehalococcoides" but share high homology in some variable regions of their 16S rRNA gene sequences. In this study, primers and PCR protocols intended to uniquely target Dehalococcoides were reevaluated, and primers and PCR protocols intended to uniquely target Dehalogenimonas were developed and tested. Use of the genus-specific primers revealed the presence of both bacterial groups in groundwater at a Louisiana Superfund site.
摘要
卤代烷脱卤属的成员与“脱氯菌属”在亲缘关系上相距较远，但在其 16S rRNA 基因序列的某些可变区具有高度同源性。在这项研究中，重新评估了旨在特异性靶向“脱氯菌属”的引物和 PCR 方案，并开发和测试了旨在特异性靶向卤代烷脱卤属的引物和 PCR 方案。使用属特异性引物表明，在路易斯安那州超级基金场地的地下水中存在这两个细菌群。
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