TF Instruments GmbH, 69120 Heidelberg, Germany.
Biotechnol Lett. 2010 Feb;32(2):249-53. doi: 10.1007/s10529-009-0135-x. Epub 2009 Oct 11.
Ultrasonic resonator technology (URT) was compared with the well established UV-Vis/ninhydrin assay to estimate protease activities in defined buffer systems. Hydrolysis of casein was measured using subtilisin, trypsin, halophilic protease from Haloferax mediterranei and Bacillus lentus alkaline protease. Sensitivity, reproducibility, working range as well as the limit of detection and the limit of quantification were comparable for both methods. Salt concentrations (0.5 M NaCl) interfered with the URT method. The quantification of protease activity by URT was possible when the product concentration measured by the UV-Vis/ninhydrin assay was correlated to the corresponding ultrasonic velocity signals.
超声谐振器技术（URT）与成熟的 UV-Vis/ninhydrin 测定法进行了比较，以评估在确定的缓冲体系中蛋白酶的活性。使用枯草杆菌蛋白酶、胰蛋白酶、来自地中海盐杆菌的嗜盐蛋白酶和地衣芽孢杆菌碱性蛋白酶测定了酪蛋白的水解。两种方法的灵敏度、重现性、工作范围以及检测限和定量限相当。盐浓度（0.5 M NaCl）会干扰 URT 方法。当通过 UV-Vis/ninhydrin 测定法测量的产物浓度与相应的超声速度信号相关联时，URT 可用于定量蛋白酶活性。
