Li Changwen, Zheng Qixin, Guo Xiaodong, Quan Daping, Zhao Jie
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Oct;29(5):592-8. doi: 10.1007/s11596-009-0512-7. Epub 2009 Oct 11.
In order to improve the surface properties of PLGA polymer for a better material/cell interface to modulate the cells behaviors, we prepared a novel three-block copolymer, PLGA-[ASP-PEG], and immobilized an RGD-containing peptide, Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) on the surface of it. Transforming growth factor-beta1 (TGF-beta1) was transfected into bone marrow stromal cells (MSCs) employed as seeded cells. Cell adhesion, spreading, proliferation and differentiation on this material were investigated. The results showed that the cell adhesive ratio on RGD-modified materials was higher than on un-modified materials (P<0.05). The extent of cell spreading was also wider on RGD-modified materials than on un-modified materials. Cell proliferation indices of transfected MSCs were increased as compared with the un-transfected MSCs (P<0.05). The ALP activities in the MSCs cultured with RGD-modified materials were higher than on un-modified materials after 14 days (P<0.05), and those in transfected MSCs were higher than in un-transfected MSCs (P<0.05). It was suggested that the combined use of RGD-modification and TGF-beta gene transfection could improve the interaction of biomaterial and cells.
为了改善聚乳酸-羟基乙酸共聚物（PLGA）聚合物的表面性能，以获得更好的材料/细胞界面来调节细胞行为，我们制备了一种新型三嵌段共聚物PLGA-[ASP-PEG]，并在其表面固定了一种含RGD的肽，即甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸-半胱氨酸（GRGDSPC）。将转化生长因子-β1（TGF-β1）转染到用作接种细胞的骨髓基质细胞（MSCs）中。研究了细胞在这种材料上的黏附、铺展、增殖和分化情况。结果表明，RGD修饰材料上的细胞黏附率高于未修饰材料（P<0.05）。RGD修饰材料上的细胞铺展程度也比未修饰材料更宽。与未转染的MSCs相比，转染的MSCs的细胞增殖指数增加（P<0.05）。培养14天后，用RGD修饰材料培养的MSCs中的碱性磷酸酶（ALP）活性高于未修饰材料（P<0.05），且转染的MSCs中的ALP活性高于未转染的MSCs（P<0.05）。提示RGD修饰与TGF-β基因转染联合使用可改善生物材料与细胞的相互作用。
