Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
Mol Biol Rep. 2010 Feb;37(2):1137-42. doi: 10.1007/s11033-009-9886-7.
A Cys2/His2-type zinc finger protein gene, DgZFP, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach. The DgZFP encodes a protein of 211 amino acids residues with a calculated molecular mass of 22.9 kDa and theoretical isoelectric point is 8.59. DgZFP contains two Cys2/His2-type zinc finger motifs, one nuclear localization domain, one Leu-rich domain, and one ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression (EAR) domain. The transcript of DgZFP was enriched in flowers than in roots, stems, and leaves of the adult chrysanthemum plants. The gene expression was strongly induced by NaCl, drought and cold treatment, and weakly by ABA treatment in the seedlings. Subcellular localization revealed that DgZFP was localized preferentially distributed to nucleus. Overexpression of DgZFP improved salt tolerance and resulted in growth suppression in transgenic tobacco. We argued that DgZFP is a new member of the Cys2/His2-type zinc finger protein genes, and it maybe function as a regulator in response to salt stress in plants.
从菊花中通过快速扩增 cDNA 末端 (RACE) 方法分离得到一个 Cys2/His2 型锌指蛋白基因 DgZFP。DgZFP 编码一个由 211 个氨基酸残基组成的蛋白质，计算分子量为 22.9 kDa，理论等电点为 8.59。DgZFP 包含两个 Cys2/His2 型锌指基序、一个核定位域、一个富含亮氨酸的结构域和一个乙烯响应元件结合因子 (ERF) 相关的双性亲脂抑制 (EAR) 结构域。DgZFP 的转录本在成年菊花植物的花朵中比在根、茎和叶中更为丰富。该基因在幼苗中受到 NaCl、干旱和寒冷处理的强烈诱导，而受到 ABA 处理的弱诱导。亚细胞定位显示 DgZFP 优先分布到细胞核。过表达 DgZFP 提高了烟草的耐盐性，并导致转基因烟草的生长受到抑制。我们认为 DgZFP 是 Cys2/His2 型锌指蛋白基因的一个新成员，它可能在植物响应盐胁迫中作为调节剂发挥作用。
