State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2022 Jul 6;13(7):1209. doi: 10.3390/genes13071209.
TFIIIA is a zinc-finger transcription factor that is involved in post-transcriptional regulation during development. Here, the gene was isolated from pak choi. Sequence analysis showed that encodes 383 amino acids (aa) with an open reading frame (ORF) of 1152 base pairs (bp). We investigated the subcellular location of BcTFIIIA and found the localized protein in the nucleus. was suppressed when the pak choi was infected by the turnip mosaic virus (TuMV). The mRNA expression level in a resistant variety was higher than that in a sensitive variety, as determined by qRT-PCR analysis. Yeast two hybrid (Y2H) assay and bimolecular fluorescence complementation (BiFC) suggested that BcTFIIIA interacts with TuMV CP and VPg in vivo, respectively, and in vitro. A virus-induced gene silencing (VIGS) experiment showed that the silencing of gene expression in pak choi promoted the accumulation of TuMV. These results suggest that BcTFIIIA negatively regulates viral infection through the interaction with TuMV CP and VPg.
TFIIIA 是一种锌指转录因子，参与发育过程中的转录后调控。本文从白菜中分离到该基因。序列分析表明，该基因编码 383 个氨基酸（aa），开放阅读框（ORF）为 1152 个碱基对（bp）。我们研究了 BcTFIIIA 的亚细胞定位，发现其定位于细胞核内。白菜感染芜菁花叶病毒（TuMV）时， 表达受到抑制。qRT-PCR 分析显示，抗性品种中的 mRNA 表达水平高于敏感品种。酵母双杂交（Y2H）和双分子荧光互补（BiFC）实验分别表明，BcTFIIIA 在体内和体外与 TuMV CP 和 VPg 相互作用。病毒诱导的基因沉默（VIGS）实验表明，白菜中 基因表达的沉默促进了 TuMV 的积累。这些结果表明，BcTFIIIA 通过与 TuMV CP 和 VPg 的相互作用，负调控病毒感染。
