Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377 Munich, Germany.
J Org Chem. 2009 Nov 6;74(21):8476-9. doi: 10.1021/jo901809k.
Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.
从固体载体上选择性释放富集的生物分子是蛋白质组学和代谢组学研究中的一个理想目标。在这里，我们证明光解光敏感的苯偶姻酯键是一种合适的替代切割策略，可用于从亲和素珠上选择性释放富集的生物分子，避免了传统热变性方法的缺点。
