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["“37摄氏度——低pH值”信号对鼠疫耶尔森氏菌细胞的影响"]

[The effect of the "37 degrees C--low pH" signal on cells of Yersinia pestis].

作者信息

Vodop'ianov S O

出版信息

Mikrobiol Zh (1978). 1990 Sep-Oct;52(5):88-92.

PMID:1982158
Abstract

It is established that the synthesis of adhesion piles registered by the results of immunoenzyme analysis and immunoblotting starts 90 min after the action on apilated cells of plaque microbe EB-76 of the signal "37 degrees C-low pH" (cultivation at 37 degrees C and acidic pH). Under these conditions a part of cell piline is in a form connected with cells and only inconsiderable part (to 20%) is represented in a form of "classical" piles possessing hemagglutinating activity. It is shown that in first 7h after the interaction of the signal "37 degrees-low pH" synthesis of adhesion piles quantitatively prevails over the synthesis of antigen of the fraction I of plaque microbe.

摘要

免疫酶分析和免疫印迹结果显示,在“37℃ - 低pH值”信号(37℃和酸性pH条件下培养)作用于去纤毛细胞后90分钟,黏附桩的合成开始。在这些条件下,一部分细胞纤毛蛋白以与细胞相连的形式存在,只有相当少的一部分(达20%)以具有血凝活性的“经典”桩的形式存在。结果表明,在“37℃ - 低pH值”信号相互作用后的最初7小时内,黏附桩的合成在数量上超过了菌斑微生物I组分抗原的合成。

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