Vodop'ianov S O
Mikrobiol Zh (1978). 1990 Sep-Oct;52(5):88-92.
It is established that the synthesis of adhesion piles registered by the results of immunoenzyme analysis and immunoblotting starts 90 min after the action on apilated cells of plaque microbe EB-76 of the signal "37 degrees C-low pH" (cultivation at 37 degrees C and acidic pH). Under these conditions a part of cell piline is in a form connected with cells and only inconsiderable part (to 20%) is represented in a form of "classical" piles possessing hemagglutinating activity. It is shown that in first 7h after the interaction of the signal "37 degrees-low pH" synthesis of adhesion piles quantitatively prevails over the synthesis of antigen of the fraction I of plaque microbe.
免疫酶分析和免疫印迹结果显示,在“37℃ - 低pH值”信号(37℃和酸性pH条件下培养)作用于去纤毛细胞后90分钟,黏附桩的合成开始。在这些条件下,一部分细胞纤毛蛋白以与细胞相连的形式存在,只有相当少的一部分(达20%)以具有血凝活性的“经典”桩的形式存在。结果表明,在“37℃ - 低pH值”信号相互作用后的最初7小时内,黏附桩的合成在数量上超过了菌斑微生物I组分抗原的合成。