Pinheiro Antonio Luiz Barbosa, Meireles Gyselle Cynthia Silva, Carvalho Carolina Montagn, Ramalho Luciana Maria Pedreira, dos Santos Jean Nunes
Laser Center, Department of Propedeutica and Clinical Integrada, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil.
Photomed Laser Surg. 2009 Dec;27(6):947-57. doi: 10.1089/pho.2009.2607.
We evaluated histologically the differences in the healing of cutaneous wounds on nourished or undernourished rats following laser phototherapy (LPT) (20 or 40 J/cm2).
Wound healing occurs due to a competitive mechanism between the synthesis and lysis of collagen. Any factor that increases the lysis or reduces the synthesis of collagen may change the healing process. Nutritional deficiencies have a great effect on wound healing.
Fifty nourished or undernourished Wistar rats had one standardized wound created on the dorsum and were divided into 10 subgroups: Control (standard diet); Control [Northeastern Brazilian basic diet (DBR)]; Standard diet+LPT (lambda635 nm; spatial average energy fluence (SAEF) 20 J/cm2); Standard diet+LPT (lambda635 nm; SAEF 40 J/cm2); Standard diet+LPT (lambda780 nm; SAEF 20 J/cm2); Standard diet+LPT (lambda780 nm; SAEF 40 J/cm2); DBR+LPT (lambda635 nm; SAEF 20 J/cm2); DBR+LPT (lambda635 nm; SAEF 40 J/cm2); DBR+LPT (lambda780 nm; SAEF 20 J/cm2); DBR+LPT (lambda780 nm; SAEF 40 J/cm2). The first application of the treatment was carried out immediately and repeated daily for seven days. The specimens were routinely processed to wax, cut, stained with H&E and Sirius Red stains, and analyzed using light microscopy. The analyses included re-epithelization, inflammatory infiltrate, and fibroblastic proliferation. Sirius Red stained slides were used to perform descriptive analyses of the collagen fibers.
The results showed that LPT with lambda635 wavelength was more effective for the treatment of undernourished subjects, treated with either LPT with lambda730 nm with SAEF of 20 J/cm2 or lambda780 nm with SAEF of 40 J/cm2.
Nutritional status influenced the progression of the healing process as well as the quality of the healed tissue. In addition, the use of both wavelengths resulted in a positive biomodulatory effect on both nourished and undernourished subjects.
我们通过组织学方法评估了激光光疗（LPT，能量密度为20或40 J/cm²）对营养良好或营养不良大鼠皮肤伤口愈合的差异。
伤口愈合是由于胶原蛋白合成与分解之间的竞争机制所致。任何增加胶原蛋白分解或减少其合成的因素都可能改变愈合过程。营养缺乏对伤口愈合有很大影响。
50只营养良好或营养不良的Wistar大鼠在背部制作一个标准化伤口，并分为10个亚组：对照组（标准饮食）；对照组[巴西东北部基本饮食（DBR）]；标准饮食+LPT（波长635 nm；空间平均能量通量（SAEF）20 J/cm²）；标准饮食+LPT（波长635 nm；SAEF 40 J/cm²）；标准饮食+LPT（波长780 nm；SAEF 20 J/cm²）；标准饮食+LPT（波长780 nm；SAEF 40 J/cm²）；DBR+LPT（波长635 nm；SAEF 20 J/cm²）；DBR+LPT（波长635 nm；SAEF 40 J/cm²）；DBR+LPT（波长780 nm；SAEF 20 J/cm²）；DBR+LPT（波长780 nm；SAEF 40 J/cm²）。首次治疗立即进行，并连续7天每日重复。标本常规处理成蜡块，切片，用苏木精-伊红（H&E）和天狼星红染色，并用光学显微镜分析。分析包括再上皮化、炎性浸润和成纤维细胞增殖。用天狼星红染色的玻片对胶原纤维进行描述性分析。
结果表明，波长635 nm的LPT对营养不良的受试者治疗效果更佳，而波长730 nm、SAEF为20 J/cm²或波长780 nm、SAEF为40 J/cm²的LPT治疗效果相对较差。
营养状况影响愈合过程的进展以及愈合组织的质量。此外，两种波长的LPT对营养良好和营养不良的受试者均产生了积极的生物调节作用。
