Esfandiari N, Kohi-Habibi M, Mosahebi Gh
Department of Plant Protection, Faculty of Horticultural Science and Plant protection Agriculture, Tehran University, Karaj, Iran.
Commun Agric Appl Biol Sci. 2006;71(3 Pt B):1281-7.
A survey was conducted to determine the incidence of Alfalfa mosaic virus (AMV), Bean yellow mosaic virus (BYMV), Broad bean wilt virus-1 (BBWV), Pea leafroll virus (PLRV), Pea enation mosaic virus (PEMV), Pea seed borne mosaic virus (PSbMV), Potato virus x(PVX), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (TSWV) on pea (Pisum sativum) in Iran. A Total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in Tehran province of Iran, where pea is grown, and tested by enzyme-linked immunosorbent assay (ELISA) using specific polyclonal antibodies. Serological diagnoses were confirmed by electron microscopy and host range studies. Incidence of viruses in decreasing order was PVX (69%), ToMV (59%), PSbMV (36.6%), BBWV-1 (26.1%), BYMV (20.3%), AMV (17.77%), TSWV (12.6%), PEMV (10.9%), PLRV (6.78%). In this survey, natural occurrence of AMV, BBWV-1, PSbMV, TSWV, PVX and ToMV was reported for the first time on the pea in Iran.
为确定苜蓿花叶病毒（AMV）、菜豆黄花叶病毒（BYMV）、蚕豆萎蔫病毒1型（BBWV）、豌豆卷叶病毒（PLRV）、豌豆耳突花叶病毒（PEMV）、豌豆种传花叶病毒（PSbMV）、马铃薯X病毒（PVX）、番茄花叶病毒（ToMV）、番茄斑萎病毒（TSWV）在伊朗豌豆（Pisum sativum）上的发病率，于2002年至2004年的春季和夏季在伊朗种植豌豆的德黑兰省共采集了1276份随机豌豆样本和684份有症状的豌豆样本，并使用特异性多克隆抗体通过酶联免疫吸附测定（ELISA）进行检测。血清学诊断通过电子显微镜和寄主范围研究得到证实。病毒发病率从高到低依次为：PVX（69%）、ToMV（59%）、PSbMV（36.6%）、BBWV-1（26.1%）、BYMV（20.3%）、AMV（17.77%）、TSWV（12.6%）、PEMV（10.9%）、PLRV（6.78%）。在本次调查中，AMV、BBWV-1、PSbMV、TSWV、PVX和ToMV在伊朗豌豆上的自然发生情况属首次报道。
