Department of Ophthalmology, Chung-Ang University Hospital, Seoul, Korea.
Artif Organs. 2010 Jan;34(1):37-45. doi: 10.1111/j.1525-1594.2009.00789.x. Epub 2009 Oct 11.
The immunogenicity of lyophilized porcine cornea is unknown. The purpose of this study was to examine the possibility of using lyophilized porcine cornea as a substrate for ocular surface reconstruction. A porcine cornea stromal button was freeze-dried and vacuum-packed. Lyophilized and fresh porcine corneas were examined histologically, and then implanted into intrastromal pockets in live rat corneas. Cytokine concentrations in plasma and protein extracts from the corneal buttons of rats were measured using the fluorokine multianalyte profiling assay, and histologic examination was performed. Immunoreactivity to the alpha-gal epitope was not found in lyophilized porcine corneas, whereas it was found in several keratocytes in fresh porcine corneas. The median survival time of rat corneas receiving lyophilized porcine transplants was 28.0 days, significantly longer than the 14.0-day survival of rat corneas that received fresh porcine transplants (P < 0.05). CD45RO(+) and CD68(+) cells were observed in rejected corneas, and interleukin-2 and interferon-gamma were elevated in rat plasma and corneal tissue. The lyophilized porcine corneal stroma, which is devoid of alpha-gal epitope, is less antigenic, and may be a useful biomaterial for ocular surface reconstruction and corneal collagen supplementation.
冻干猪角膜的免疫原性尚不清楚。本研究旨在探讨将冻干猪角膜作为眼表重建的基质的可能性。将猪角膜基质纽扣冻干并真空包装。对冻干和新鲜猪角膜进行组织学检查，然后将其植入活鼠角膜的基质袋中。使用荧光素酶多分析物分析试剂盒测量来自大鼠角膜纽扣的血浆和蛋白提取物中的细胞因子浓度，并进行组织学检查。在冻干的猪角膜中未发现针对α-gal 表位的免疫反应性，而在新鲜的猪角膜中发现了几个角膜细胞。接受冻干猪移植物的大鼠角膜的中位存活时间为 28.0 天，明显长于接受新鲜猪移植物的大鼠角膜的 14.0 天存活时间（P<0.05）。在排斥的角膜中观察到 CD45RO(+) 和 CD68(+) 细胞，大鼠血浆和角膜组织中白细胞介素-2 和干扰素-γ升高。不含 α-gal 表位的冻干猪角膜基质的抗原性较低，可能是眼表重建和角膜胶原补充的有用生物材料。
