Moore M B, Gebhardt B M, Verity S M, McDonald M B
Invest Ophthalmol Vis Sci. 1987 Mar;28(3):555-9.
The antigenicity of intrastromal and epikeratophakia xenografts of lyophilized corneal tissue was evaluated in nonimmune and immune recipients. Lyophilized feline lenticules were implanted into intrastromal pockets in unsensitized rabbits and rabbits sensitized to the donor cat. In both cases, the grafts remained clear. Sensitized rabbits with clear intrastromal grafts received fresh tissue penetrating keratoplasty grafts from the same donor cat, placed adjacent to the intrastromal grafts. The fresh tissue penetrating keratoplasty grafts were rapidly rejected, while the lyophilized intrastromal grafts remained clear. Cats sensitized to rabbits received lyophilized and rehydrated epikeratophakia grafts shaped from rabbit cornea; these lyophilized grafts also remained clear for the 3-month period of the study. The results indicate that lyophilized and rehydrated corneal stroma, which is devoid of living cells, is not antigenic and is not subjected to immunologic attack, even in cases where the donor and host are of different species and the host has been previously immunized to the donor.
在非免疫和免疫受体中评估了冻干角膜组织的基质内和角膜表面镜片植入异种移植物的抗原性。将冻干的猫晶状体植入未致敏的兔子以及对供体猫致敏的兔子的基质袋中。在这两种情况下，移植物均保持透明。基质内移植物透明的致敏兔子接受了来自同一供体猫的新鲜组织穿透性角膜移植移植物，放置在基质内移植物旁边。新鲜组织穿透性角膜移植移植物迅速被排斥，而冻干的基质内移植物仍保持透明。对兔子致敏的猫接受了由兔角膜制成的冻干并复水的角膜表面镜片移植；在研究的3个月期间，这些冻干移植物也保持透明。结果表明，即使供体和宿主属于不同物种且宿主先前已对供体免疫，缺乏活细胞的冻干并复水的角膜基质也没有抗原性，不会受到免疫攻击。
