鸽（家鸽）背根神经节中囊泡谷氨酸转运体2信使核糖核酸的定位

Localization of vesicular glutamate transporter 2 mRNA in the dorsal root ganglion of the pigeon (Columba livia).

作者信息

Atoji Y, Islam M R

机构信息

Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.

出版信息

Anat Histol Embryol. 2009 Nov;38(6):475-8. doi: 10.1111/j.1439-0264.2009.00978.x. Epub 2009 Oct 12.

DOI:10.1111/j.1439-0264.2009.00978.x

PMID:19821818

Abstract

Our previous study showed localization of glutamate receptor 1 (GluR1) mRNA in neurons of the pigeon spinal cord, suggesting glutamatergic input from intrinsic and extrinsic origins. The present study examined localization of vesicular glutamate transporter 2 (VGLUT2) mRNA to confirm an extrinsic origin of glutamatergic neurons in the dorsal root ganglion (DRG). GluR1 and GluR2 mRNAs were examined in DRG and spinal cord to seek projection regions from VGLUT2 mRNA-expressing neurons. VGLUT2 mRNA was expressed in most DRG neurons and labelling intensity varied from weakly to intensely. Intense VGLUT2 mRNA expression was mainly seen in medium to large neurons. GluR1 and GluR2 mRNAs were expressed in the dorsal horn and GluR2 mRNA signal was also seen in the marginal nucleus. The results suggest that the pigeon DRG has an extrinsic glutamatergic origin that project to the dorsal horn and marginal nucleus of the spinal cord.

摘要

我们之前的研究表明，谷氨酸受体1（GluR1）mRNA定位于鸽子脊髓的神经元中，提示存在来自内在和外在来源的谷氨酸能输入。本研究检测了囊泡谷氨酸转运体2（VGLUT2）mRNA的定位，以证实背根神经节（DRG）中谷氨酸能神经元的外在来源。检测了DRG和脊髓中的GluR1和GluR2 mRNA，以寻找表达VGLUT2 mRNA的神经元的投射区域。VGLUT2 mRNA在大多数DRG神经元中表达，标记强度从弱到强不等。强烈的VGLUT2 mRNA表达主要见于中大型神经元。GluR1和GluR2 mRNA在背角表达，在边缘核中也可见GluR2 mRNA信号。结果表明，鸽子DRG具有投射至脊髓背角和边缘核的外在谷氨酸能起源。