细胞间黏附分子-1第6外显子（E469K）的多态性与结直肠癌的分化相关。
Polymorphisms of the ICAM-1 exon 6 (E469K) are associated with differentiation of colorectal cancer.
作者信息
Wang Qing-lei, Li Bing-hui, Liu Bin, Liu Ya-bin, Liu Yue-Ping, Miao Sui-Bing, Han Yi, Wen Jin-Kun, Han Mei
机构信息
Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, China.
出版信息
J Exp Clin Cancer Res. 2009 Oct 13;28(1):139. doi: 10.1186/1756-9966-28-139.
BACKGROUND
Genetic factors are thought to play a role in development for colorectal carcinogenesis. ICAM-1 is a polymorphic gene, thus, the present study investigated the relationship between the polymorphisms of ICAM-1 and the susceptibility and phenotypical characteristics of colorectal cancer (CRC).
METHODS
The polymorphisms at ICAM-1 exon 4 (G241R) and exon 6 (E469K) were detected by PCR with sequence-specific primers. The relationship between specific genotypes of ICAM-1 and differentiation of CRC was evaluated by the histological grade.
RESULTS
We showed only GG genotype of ICAM-1 individuals in either CRC or normal controls. The KK genotype of ICAM-1 K469E was found more frequently than in the controls (P < 0.05). Patients with well-differentiated CRC displayed the KK more frequently than those of poor differentiation (P < 0.05).
CONCLUSION
The findings indicate that polymorphisms of G241R are rare in Chinese population and that KK genotype of ICAM-1 K469E is significantly associated with well differentiation of CRC.
背景
遗传因素被认为在结直肠癌发生发展中起作用。细胞间黏附分子-1（ICAM-1）是一个多态性基因，因此，本研究调查了ICAM-1基因多态性与结直肠癌（CRC）易感性及表型特征之间的关系。
方法
采用序列特异性引物PCR法检测ICAM-1基因第4外显子（G241R）和第6外显子（E469K）的多态性。通过组织学分级评估ICAM-1特定基因型与CRC分化程度之间的关系。
结果
在CRC患者和正常对照中，我们仅发现ICAM-1个体的GG基因型。ICAM-1 K469E的KK基因型在患者中的发现频率高于对照组（P < 0.05）。高分化CRC患者中KK基因型的出现频率高于低分化患者（P < 0.05）。
结论
研究结果表明，G241R多态性在中国人群中罕见，且ICAM-1 K469E的KK基因型与CRC的高分化显著相关。
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