Wang K, Chen Y, Liu S, Qiu S, Gao S, Huang X, Zhang J, Peng X, Qiani W, Zhang J Y
Department of Biological Sciences, The University of Texas at El Paso, El Paso, Texas 79968, USA.
Int J Immunopathol Pharmacol. 2009 Jul-Sep;22(3):735-43. doi: 10.1177/039463200902200319.
In order to understand the immunogenicity of a tumor-associated antigen (TAA), Ras family small GTP binding protein (Ra1A) in hepatocellular carcinoma (HCC), autoantibody responses to RalA were evaluated by enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay in sera from patients with HCC and sera from normal individuals. Immunohistochemistry (IHC) study with tissue array slides was also performed to analyze protein expression profiles of RalA in HCC and control tissues. This study demonstrated that RalA had a relative higher frequency of autoantibody response in HCC (20.1%) compared to liver cirrhosis (3.3%), chronic hepatitis (0%), and normal individuals sera (0%). RalA also showed a stepwise increased expression from normal liver tissues (26.7%), liver cirrhosis tissues (45.0%) to HCC tissues (63.3%). Sensitivity and specificity of anti-RalA antibody in detection of HCC was 20.1% and 99.3%, respectively. The data suggested that RalA might contribute to liver malignant transformation, and could be used as a potential tumor marker in HCC detection.
为了解肿瘤相关抗原（TAA）——Ras家族小GTP结合蛋白（RalA）在肝细胞癌（HCC）中的免疫原性，采用酶联免疫吸附测定（ELISA）、蛋白质印迹法和间接免疫荧光测定法，对HCC患者血清和正常个体血清中针对RalA的自身抗体反应进行了评估。还利用组织芯片载玻片进行了免疫组织化学（IHC）研究，以分析RalA在HCC组织和对照组织中的蛋白表达谱。本研究表明，与肝硬化（3.3%）、慢性肝炎（0%）和正常个体血清（0%）相比，RalA在HCC中的自身抗体反应频率相对较高（20.1%）。RalA的表达也呈现出从正常肝组织（26.7%）、肝硬化组织（45.0%）到HCC组织（63.3%）逐步升高的趋势。抗RalA抗体检测HCC的敏感性和特异性分别为20.1%和99.3%。这些数据表明，RalA可能促成肝脏恶性转化，并可作为HCC检测中的一种潜在肿瘤标志物。
