用具有聚集诱导发射的无标记荧光探针 silole 对 G-四链体 DNA 进行可视化观察。

Visual observation of G-quadruplex DNA with the label-free fluorescent probe silole with aggregation-induced emission.

机构信息

College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan 430072, PR China.

出版信息

Bioorg Med Chem. 2009 Nov 15;17(22):7743-8. doi: 10.1016/j.bmc.2009.09.040. Epub 2009 Sep 25.

DOI:10.1016/j.bmc.2009.09.040

PMID:19822430

Abstract

AIE molecule silole 1 could be used to detect G-quadruplex formation using an exonuclease I hydrolysis assay. This visual observation of G-quadruplexes has been successfully used in investigating multiple G-quadruplexes, including the one-stranded telomeric, c-myc, c-kit, VEGF G-quadruplexes, and a d(G(4)T(4)G(4)) inter-molecular G-quadruplex. The detection of G-quadruplex can be also applied to G-quadruplex isomers induced by small molecules.

摘要

AIE 分子 silole 1 可以用于使用外切酶 I 水解分析来检测 G-四链体的形成。这种对 G-四链体的可视化观察已成功应用于研究多种 G-四链体，包括单链端粒、c-myc、c-kit、VEGF G-四链体和 d(G(4)T(4)G(4)) 分子间 G-四链体。G-四链体的检测也可以应用于小分子诱导的 G-四链体异构体。