Akbay Nuriye, Mahdavi Farhad, Lakowicz Joseph R, Ray Krishanu
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD, 21201, USA.
Chem Phys Lett. 2012 Oct 1;548:45-50. doi: 10.1016/j.cplett.2012.08.020.
We investigated the feasibility of using platinum nanostructures to accomplish the metal-enhanced fluorescence (MEF) in the UV spectral region. We examine the possibility for detection of the intrinsic fluorescence from nucleotides and G-quadruplex DNA on platinum nanoparticles. Guanosine monophosphate (GMP) showed significant increases (~20-fold) in fluorescence intensities in the presence of platinum nanostructures when compared to quartz controls. G-quadruplex DNA demonstrated ~5-fold increase in fluorescence intensity and higher photostability in the presence of Pt nanostructures. We performed finite element method simulations to explore how Pt nanoparticles interact with plane waves and conformed that the Pt nanostructures are promising for enhancing the fluorescence emission in the UV region.
我们研究了使用铂纳米结构在紫外光谱区域实现金属增强荧光（MEF）的可行性。我们考察了检测铂纳米颗粒上核苷酸和G-四链体DNA固有荧光的可能性。与石英对照相比，在存在铂纳米结构的情况下，单磷酸鸟苷（GMP）的荧光强度显著增加（约20倍）。在存在铂纳米结构的情况下，G-四链体DNA的荧光强度增加了约5倍，且光稳定性更高。我们进行了有限元方法模拟，以探索铂纳米颗粒如何与平面波相互作用，并证实铂纳米结构有望增强紫外区域的荧光发射。
