持续性非卧床腹膜透析（CAPD）患者的腹膜巨噬细胞在腹膜炎期间分泌白细胞介素-1β的能力增强。

Peritoneal macrophages from patients on CAPD show an increased capacity to secrete interleukin-1 beta during peritonitis.

作者信息

Fieren M W, van den Bemd G J, Bonta I L

机构信息

Department of Internal Medicine (I), University Hospital Dijkzigt, Erasmus University Rotterdam.

出版信息

Adv Perit Dial. 1990;6:120-2.

PMID:1982787

Abstract

The in vitro release of interleukin-1 beta (IL-1 beta) by peritoneal macrophages from CAPD patients was studied during 16 infection free periods (16 patients) and 13 episodes of peritonitis (10 patients) using an ELISA. Without exogeneous stimulation with LPS, peritoneal macrophages released the same amounts of IL-1 beta. irrespective if they were obtained during an infection free period (473 +/- 92 pg/ml 24h, means +/- SEM) or during peritonitis (324 +/- 125 pg/ml). However, in response to a dose of 5 micrograms/ml of LPS, peritoneal macrophages released significantly more (p less than 0.005) IL-1 beta during peritonitis (6155 +/- 1743 pg/ml). These findings show that during peritonitis, peritoneal macrophages are primed in vivo to release more IL-1 beta in vitro after stimulation with LPS, indicating that activation of peritoneal macrophages for IL-1 beta secretion occurs stepwise.

摘要

采用酶联免疫吸附测定法（ELISA），对16例无感染期（16名患者）和13例腹膜炎发作期（10名患者）持续性不卧床腹膜透析（CAPD）患者腹膜巨噬细胞白细胞介素-1β（IL-1β）的体外释放情况进行了研究。在无脂多糖（LPS）外源刺激的情况下，腹膜巨噬细胞释放的IL-1β量相同，无论这些细胞是在无感染期（24小时为473±92皮克/毫升，均值±标准误）还是在腹膜炎期间（324±125皮克/毫升）获取的。然而，在对5微克/毫升的LPS剂量作出反应时，腹膜炎期间腹膜巨噬细胞释放的IL-1β显著更多（p<0.005）（6155±1743皮克/毫升）。这些发现表明，在腹膜炎期间，腹膜巨噬细胞在体内被致敏，在用LPS刺激后体外释放更多的IL-1β，这表明腹膜巨噬细胞IL-1β分泌的激活是逐步发生的。