Department of Ophthalmology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo 113-8431, Japan.
Br J Ophthalmol. 2010 Apr;94(4):513-8. doi: 10.1136/bjo.2009.165316. Epub 2009 Oct 14.
The essential role of basophils as an initiator of chronic allergic reaction has been elucidated in mouse models. The aim of this present study was to analyse the in situ immunolocalisation of basophils and other relevant inflammatory cells in chronic allergic keratoconjunctivitis.
Transmission electron microscopic (TEM) analysis was carried out to examine the existence of basophils in the giant papillae obtained from atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) patients. Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-FcepsilonRI-beta antibodies.
TEM analysis confirmed the existence of basophils in the giant papillae. Small clusters of basophils were observed in the substantia propria of giant papillae, especially at the vicinity of vascular endothelium and subepithelial regions. BB-1-positive basophil clusters were surrounded by T cells, B cells, IgE-positive cells and MBP-positive eosinophils. No BB-1-positive basophils were observed in the control conjunctivae.
Basophils may infiltrate from either vascular endothelium into the giant papillae. The existence of basophils at the centre of inflammatory cells suggests the role of basophils as an initiator of chronic allergic conjunctivitis.
在小鼠模型中阐明了嗜碱性粒细胞作为慢性过敏反应启动者的基本作用。本研究旨在分析慢性过敏性角结膜炎中嗜碱性粒细胞和其他相关炎症细胞的原位免疫定位。
通过透射电子显微镜（TEM）分析，检查来自特应性角结膜炎（AKC）和春季角结膜炎（VKC）患者的巨大乳头中是否存在嗜碱性粒细胞。用嗜碱性粒细胞特异性抗体 BB-1 和抗 CD4、抗 CD8、抗 CD20、抗主要碱性蛋白（MBP）、抗 IgE 和抗 FcepsilonRI-beta 抗体对巨大乳头的冷冻切片进行免疫染色。
TEM 分析证实了巨大乳头中嗜碱性粒细胞的存在。在巨大乳头的固有层中观察到嗜碱性粒细胞的小簇，特别是在血管内皮附近和上皮下区域。BB-1 阳性嗜碱性粒细胞簇被 T 细胞、B 细胞、IgE 阳性细胞和 MBP 阳性嗜酸性粒细胞包围。在对照结膜中未观察到 BB-1 阳性嗜碱性粒细胞。
嗜碱性粒细胞可能从血管内皮渗透到巨大乳头中。炎症细胞中心存在嗜碱性粒细胞提示嗜碱性粒细胞作为慢性过敏性结膜炎的启动者的作用。
