肾组织冷应激诱导肿胀后容量恢复的比较研究——II. 呋塞米、阿米洛利及钾通透性对兔皮质切片的影响

Comparative studies of volume restoration following cold-stress induced swelling in renal tissues--II. Effects of furosemide, amiloride and K+ permeability in rabbit cortex slices.

Ouahbi A, Gilles R

Laboratory of animal physiology, University of Liège, Belgique.

Comp Biochem Physiol A Comp Physiol. 1990;97(2):275-8. doi: 10.1016/0300-9629(90)90184-t.

Abstract

Inhibition of volume restoration by ouabain in rabbit kidney cortex slices is related to an increase in Na+ concentration which may reach levels higher than equilibrium with the external medium. 2. The uphill accumulation of Na+ is not sensitive to amiloride and furosemide. It is dependent on the level of K+ in the external medium. 3. Bringing the slices from cold to normal temperature induces a large increase in Rb86 efflux. 4. It is concluded that the Na+ entry inhibiting volume restoration is driven by an increase in K+ expel occurring upon tissue rewarming.

摘要

哇巴因对兔肾皮质切片体积恢复的抑制作用与钠离子浓度升高有关，该浓度可能高于与外部介质的平衡水平。2. 钠离子的逆浓度梯度积累对氨氯吡脒和速尿不敏感。它取决于外部介质中的钾离子水平。3. 将切片从低温恢复到常温会导致铷86外流大幅增加。4. 得出的结论是，抑制体积恢复的钠离子内流是由组织复温时钾离子排出增加所驱动的。