Zhao Xue Mei, Ye Xing Qian, Zhu Da Yuan
Pharmacy College, Taishan Medical University, Taian 271016, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2009 Oct 18;41(5):575-7.
To study the chemical constituents of the peels collected from Citrus changshan-huyou Y. B. Chang, and further screen the bioactive components as the lead structures.
These compounds were isolated by repeated flash column chromatography on silica gel and Sephadex LH-20. The structures of isolated compounds were elucidated by using IR, EIMS, and NMR analyses.
One novel compound along with nine known compounds were obtained and identified as huyoujiasu(I), 3-hydroxy-4-methoxybenzoic acid(II), 3, 4-dihydroxybenzoic acid(III), fatty acid(IV), glyceride(V), 6, 7-dimethoxycoumarin(VI), 6', 7'-dihydroxybergamottin(VII), daucossterol A(VIII), daucossterol B(IX) and huyou-triterpenoid(X).
One new compound, Huyoujiasu, was obtained from these peels by repeated column chromatography, and the other known compounds were isolated from this fruit for the first time as well.
研究常山胡柚（Citrus changshan-huyou Y. B. Chang）果皮的化学成分，并进一步筛选具有生物活性的成分作为先导结构。
通过硅胶柱反复快速柱色谱和葡聚糖凝胶LH-20柱色谱分离这些化合物。利用红外光谱（IR）、电子轰击质谱（EIMS）和核磁共振（NMR）分析对分离得到的化合物结构进行鉴定。
得到一个新化合物和九个已知化合物，分别鉴定为胡柚甲素（I）、3-羟基-4-甲氧基苯甲酸（II）、3,4-二羟基苯甲酸（III）、脂肪酸（IV）、甘油酯（V）、6,7-二甲氧基香豆素（VI）、6',7'-二羟基佛手柑内酯（VII）、胡萝卜苷A（VIII）、胡萝卜苷B（IX）和胡柚三萜（X）。
通过反复柱色谱从这些果皮中得到一个新化合物胡柚甲素，其他已知化合物也是首次从该果实中分离得到。
