Michalaki H, Nicolas J F, Kanitakis J, Machado P, Roche P, Thivolet J
Clinique Dermatologique, INSERM U.209, Hopital Edouard-Herriot, Lyon, France.
Reg Immunol. 1990;3(3):151-5.
Bullous pemphigoid (BP) is an auto-immune blistering skin disease associated with the presence of autoantibodies directed to BP antigens located at the basement membrane zone of the dermal-epidermal junction. The present study was designed to test for the involvement of cellular immune mechanisms in the pathogenesis of blister formation. An immunohistochemical analysis of the lesional, peri-lesional, and pre-lesional mononuclear cell infiltrates, performed in 7 BP patients, revealed the following observations: 1) all the skin biopsies contained a high percentage of mononuclear cells within the inflammatory cellular infiltrate; 2) T cells (CD3+, CD45RO+), with a phenotype of activated helper T cell (CD4+, CD25+) were consistently found; 3) T cells were found beneath the bullae but, more importantly, also at the dermal-epidermal junction in close contact with the basement membrane zone in pre- and peri-bullous lesions. These results show that activated T cells are found at the early phase of the onset of BP lesions and suggest that they could play a role, in association with autoantibodies, in the pathogenesis of bulla formation.
大疱性类天疱疮（BP）是一种自身免疫性水疱性皮肤病，与针对位于真皮 - 表皮交界处基底膜带的BP抗原的自身抗体有关。本研究旨在检测细胞免疫机制在水疱形成发病机制中的作用。对7例BP患者的皮损、皮损周围和皮损前单核细胞浸润进行免疫组织化学分析，结果如下：1）所有皮肤活检标本的炎性细胞浸润中单核细胞比例均较高；2） consistently发现具有活化辅助性T细胞表型（CD4 +、CD25 +）的T细胞（CD3 +、CD45RO +）；3）在水疱下方发现T细胞，但更重要的是，在水疱前和水疱周围皮损的真皮 - 表皮交界处也发现T细胞与基底膜带紧密接触。这些结果表明，在BP皮损发病早期发现了活化的T细胞，提示它们可能与自身抗体一起在水疱形成的发病机制中起作用。
