Schaller J, Haustein U F
Klinik und Poliklinik für Hautkrankheiten, Karl-Marx-Universität Leipzig.
Hautarzt. 1989 May;40(5):296-303.
In 35 patients with bullous pemphigoid (BP) various parameters of the cellular immune system were studied. It was shown that the average 29% decrease in the number rosette-forming lymphocytes in sheep red blood cells is converted by the immune complexes and by a second serum factor in the acute stage of BP that has not yet been characterized precisely. These changes were reversible in patients who were placed on immunosuppressants. Obviously, this mechanism is partly due to the binding of immune complexes via Fc receptors on T-lymphocytes and is consequently due to the steral blocking of sheep erythrocyte receptors. No decrease in the number of T-cells was found by means of monoclonal antibodies (CD2, CD3), and the number of suppressor cells was also normal (CD8). On the other hand, the number of T-helper cells increased by an average of 15% (CD4). In addition, in the acute stage, there is a significant increase in the number of activation antigen Tac (CD25) and DR-bearing lymphocytes: 29% and 37% respectively (the normal values are 2% and 11%, respectively). Double staining permitted the phenotype of activated lymphocytes to be detected within the T-cells and the T-helper cell subpopulation, respectively. The number of activated T-helper cells correlates with the disease activity, and from that point it seems to be useful as a marker of activity in follow-up studies.
对35例大疱性类天疱疮（BP）患者的细胞免疫系统各项参数进行了研究。结果显示，在BP急性期，免疫复合物和一种尚未精确鉴定的第二种血清因子可使与绵羊红细胞形成玫瑰花结的淋巴细胞数量平均减少29%。这些变化在接受免疫抑制剂治疗的患者中是可逆的。显然，这种机制部分归因于免疫复合物通过T淋巴细胞上的Fc受体结合，进而导致绵羊红细胞受体的空间位阻。通过单克隆抗体（CD2、CD3）未发现T细胞数量减少，抑制细胞数量（CD8）也正常。另一方面，T辅助细胞数量平均增加了15%（CD4）。此外，在急性期，活化抗原Tac（CD25）和携带DR的淋巴细胞数量显著增加：分别增加了29%和37%（正常值分别为2%和11%）。双重染色分别在T细胞和T辅助细胞亚群中检测到活化淋巴细胞的表型。活化T辅助细胞的数量与疾病活动度相关，从这一点来看，它似乎可作为随访研究中疾病活动度的一个有用标志物。
