Ogbe A O, Atawodi S E, Abdu P A, Sannusi A, Itodo A E
Federal College of Animal Health and Production Technology, National Veterinary Research Institute, Vom, Nigeria.
J S Afr Vet Assoc. 2009 Jun;80(2):97-102. doi: 10.4102/jsava.v80i2.179.
An experiment was conducted to study the effect of a wild Ganoderma lucidum aqueous extract in coccidian-infected broilers. At 6 weeks of age the birds were randomly allocated to 6 treatment groups of 20 Ross broilers each in wire cages. Groups A, B and C were infected with Eimeria tenella Houghton strain at the rate of 36 250 sporulated oocysts/ml per bird. The remaining 3 groups D, E and F were uninfected controls. At 7 weeks the birds in group A were treated with G. lucidum aqueous extract and those in B with amprolium in drinking water ad libitum at the rate of 200 mg/ml each for 7 days consecutively. Body weight gain, feed intake, faecal oocyst output and some haematological parameters were monitored. The result showed that all the infected birds in groups A, B and C had clinical signs of weakness and reduced appetite on day 4 post-infection. By the 5th day post-infection their faeces became bloody and watery, and large numbers of E. tenella oocysts were present in the faeces. On day 3 after treatment the oocysts detected were considerably reduced in both treated groups A and B and slightly higher in the untreated group C. The faeces of the uninfected control groups were normal and free of coccidial oocysts. After treatment for 7 days no coccidial oocysts were found in faeces of the birds that had been treated. Infected, untreated birds showed a slight drop in feed intake and weight gain from 7 to 8 weeks of age. The final mean weight gain recorded in the treated groups A and B was comparable to that of the uninfected birds in the 3 control groups, while it was lower in the untreated group C. The feed to gain ratio was higher in C than in the other groups. A slight drop in packed cell volume was observed in groups A, B and C at 7 weeks of age, 1 week after infection. This study showed that treatment with G. lucidum results in a marked reduction in the number of E. tenella oocysts shed in the faeces, leading to improved weight gain and decreased weight loss. The results confirmed the virulence of the Houghton strain of E. tenella and the effectiveness of both amprolium and G. lucidum extract against E. tenella.
进行了一项实验，以研究野生灵芝水提取物对感染球虫的肉鸡的影响。6周龄时，将这些鸡随机分配到6个处理组，每组20只罗斯肉鸡，饲养在铁丝笼中。A、B和C组每只鸡以36250个孢子化卵囊/毫升的剂量感染柔嫩艾美耳球虫霍顿株。其余3组D、E和F为未感染的对照组。7周时，A组鸡用灵芝水提取物处理，B组鸡在饮水中自由添加氨丙啉，剂量均为200毫克/毫升，连续处理7天。监测体重增加、采食量、粪便卵囊产量和一些血液学参数。结果显示，A、B和C组所有感染鸡在感染后第4天出现虚弱和食欲减退的临床症状。感染后第5天，它们的粪便变得带血且呈水样，粪便中存在大量柔嫩艾美耳球虫卵囊。治疗后第3天，A组和B组检测到的卵囊数量大幅减少，未治疗的C组略高。未感染对照组的粪便正常，无球虫卵囊。治疗7天后，接受治疗的鸡的粪便中未发现球虫卵囊。感染但未治疗的鸡在7至8周龄时采食量和体重增加略有下降。A组和B组治疗后的最终平均体重增加与3个对照组中未感染鸡的相当，而未治疗的C组较低。C组的料肉比高于其他组。感染后1周，即7周龄时，A、B和C组的红细胞压积略有下降。本研究表明，用灵芝治疗可显著减少粪便中排出的柔嫩艾美耳球虫卵囊数量，从而改善体重增加并减少体重减轻。结果证实了柔嫩艾美耳球虫霍顿株的毒力以及氨丙啉和灵芝提取物对柔嫩艾美耳球虫的有效性。
