Wang Dingfa, Zhou Luli, Li Wei, Zhou Hanlin, Hou Guanyu
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, China.
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, China; College of Agriculture, Hainan University, Haikou, Hainan, 570228, China.
Exp Parasitol. 2018 Jan;184:16-21. doi: 10.1016/j.exppara.2017.11.002. Epub 2017 Nov 14.
To study the anticoccidial effects of areca nut extract (ANE) on coccidiosis, 270 one-day old Wenchang broiler chicks were divided into six equal groups, each with three replicate cages (n = 15 per cage). The six groups were the blank control group (BC), negative control group (NC), positive control group (PC), and three ANE-treated groups. The birds in the three control groups (BC, NC and PC) were fed a basal diet without ANE supplementation. The birds in the three ANE-treated groups were fed a basal diet supplied with ANE at 100 (T1), 200 (T2), or 300 (T3) mg/kg feed. At 15 days of age, the birds in the NC, PC and the three ANE groups were challenged orally with 1 × 10Eimeria tenella oocysts per chick. At 48 h after oocysts inoculation, the birds in group PC were supplied diclazuril with drinking water for 5 days. The results showed that ANE and diclazuril significantly improved feed intake and body weight gain (P < 0.05) relative to the NC group. Both ANE and diclazuril significantly (P < 0.05) reduced OPG on day 4-9 post-inoculation (p.i.) relative to the NC group. Coccidial infection damaged the integrity of the cecal mucosa and thickened cecal tunica muscularis. ANE and diclazuril mitigated the mucosal damage caused by coccidial infection. Diet ANE supplementation reduced the cecal lesion scores compared to the NC group (P < 0.05). ANE and diclazuril increased nitric oxide (NO) levels at 3 days p.i., but reduced NO levels at 6 days p.i. (P < 0.05) compared to the NC group. Diet ANE supplementation increased the concentration of interleukin 2 (IL-2) in infected chicken relative to the NC group. The current results showed the anticoccidial properties, and beneficial effect on intestinal mucosa damage of ANE in broiler chicks challenged with coccidiosis.
为研究槟榔提取物（ANE）对球虫病的抗球虫作用，将270只1日龄文昌肉鸡雏鸡分为6个相等的组，每组有3个重复笼（每个笼n = 15）。这6组分别为空白对照组（BC）、阴性对照组（NC）、阳性对照组（PC）和3个ANE处理组。3个对照组（BC、NC和PC）的鸡饲喂不添加ANE的基础日粮。3个ANE处理组的鸡饲喂添加了100（T1）、200（T2）或300（T3）mg/kg饲料ANE的基础日粮。在15日龄时，NC组、PC组和3个ANE组的鸡每只口服接种1×10个柔嫩艾美耳球虫卵囊。在接种卵囊后48小时，PC组的鸡饮用含地克珠利的水5天。结果表明，与NC组相比，ANE和地克珠利显著提高了采食量和体重增加（P < 0.05）。在接种后第4 - 9天（p.i.），ANE和地克珠利均相对于NC组显著（P < 0.05）降低了每克粪便卵囊数（OPG）。球虫感染破坏了盲肠黏膜的完整性并使盲肠肌层增厚。ANE和地克珠利减轻了球虫感染引起的黏膜损伤。与NC组相比，日粮中添加ANE降低了盲肠病变评分（P < 0.05）。与NC组相比，ANE和地克珠利在接种后3天增加了一氧化氮（NO）水平，但在接种后6天降低了NO水平（P < 0.05）。日粮中添加ANE相对于NC组增加了感染鸡体内白细胞介素2（IL - 2）的浓度。目前的结果显示了ANE在感染球虫病的肉鸡雏鸡中的抗球虫特性以及对肠道黏膜损伤的有益作用。
