Malicki K, Malicka E, Bańbura M W, Niemiałtowski M, Ladyńska A
Department of Microbiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.
Acta Virol. 1990 Dec;34(6):523-8.
Rotaviruses were isolated in primary bovine foetal kidney cells from 3 of 13 (23.1%) tested suckling calves and from 3 of 14 (21.4%) suckling piglets both suffering of acute diarrhoea, as confirmed by serological tests. The presence of rotaviral antigen in culture supernatant was revealed by double sandwich enzyme immunoassay (EIA) and direct immunofluorescence (IF). Transmission electron microscopy (TEM) and immune electron microscopy (IEM) showed particles with an average diameter of 70 nm. The strains adapted to continuous pig kidney cells PK-15 have lost their reactivity in EIA, although TEM revealed the presence of rotavirions.
从13头接受检测的患急性腹泻的哺乳犊牛中的3头（23.1%）以及14头患急性腹泻的哺乳仔猪中的3头（21.4%）的原代牛胎儿肾细胞中分离出轮状病毒，血清学检测证实了这一点。通过双夹心酶免疫测定法（EIA）和直接免疫荧光法（IF）揭示了培养上清液中轮状病毒抗原的存在。透射电子显微镜（TEM）和免疫电子显微镜（IEM）显示颗粒的平均直径为70纳米。适应连续猪肾细胞PK - 15的毒株在EIA中失去了反应性，尽管TEM显示存在轮状病毒粒子。
