Sigolo de San Juan C, Bellinzoni R C, Mattion N, La Torre J, Scodeller E A
Res Vet Sci. 1986 Sep;41(2):270-2.
The incidence of rotaviruses as a gastroenteritis causal agent in piglets was studied in 19 pig herds of Sao Paulo State, Brazil, during 1985. From 302 diarrhoea samples collected during January (summer), 65 were positive for rotavirus when analysed by enzyme-linked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE). Sixty-two of these samples belonged to the classical group A rotavirus, three to atypical rotaviruses (ELISA negative and probably group B) and one elicited a mixed electropherotype of group A and atypical rotavirus and was ELISA positive. Atypical viruses appear to be very fragile and were rapidly degraded upon storage of samples at -20 degrees C. Three herds where atypical rotaviruses were present in January were sampled again in August (winter). Nine atypical isolates out of a total 21 positive samples (assayed by electron microscopy and PAGE) were detected again in two of them.
1985年，在巴西圣保罗州的19个猪群中研究了轮状病毒作为仔猪肠胃炎病原体的发病率。1月（夏季）收集的302份腹泻样本，经酶联免疫吸附测定（ELISA）和聚丙烯酰胺凝胶电泳（PAGE）分析，65份轮状病毒呈阳性。其中62份样本属于经典的A组轮状病毒，3份属于非典型轮状病毒（ELISA阴性，可能为B组），1份呈现A组和非典型轮状病毒的混合电泳型，且ELISA呈阳性。非典型病毒似乎非常脆弱，样本在-20℃储存时会迅速降解。1月存在非典型轮状病毒的三个猪群在8月（冬季）再次采样。其中两个猪群在总共21份阳性样本（通过电子显微镜和PAGE检测）中再次检测到9份非典型分离株。
