用于口蹄疫疫苗生产的悬浮培养。细胞、病毒和疫苗的评估标准。

The use of suspension cultures for FMD vaccine production. Criteria for the evaluation of cells, virus and vaccine.

作者信息

Nardelli L, Panina G F

出版信息

Dev Biol Stand. 1976;35:9-25.

PMID:198318

Abstract

A survey of problems connected with the cell suspension culture for the production of FMD vaccine is presented, in order to stimulate a discussion. Introductory remarks are made on the following topics: 1) suspension cell lines used for FMD vaccine production, 2) terminology, 3) criteria for the characterization of a cell culture, 4) evaluation of FMD virus produced according to the cell suspension technique and correlation existing between in vitro tests and antigenicity in domestic animals, 5) criteria which must be met by FMD vaccine produced from virus cultivated on cell suspension.

摘要

本文介绍了与口蹄疫疫苗生产中的细胞悬浮培养相关问题的调查情况，以引发讨论。就以下主题作了初步论述：1）用于口蹄疫疫苗生产的悬浮细胞系；2）术语；3）细胞培养特性的标准；4）根据细胞悬浮技术生产的口蹄疫病毒的评估以及体外试验与家畜抗原性之间存在的相关性；5）由在细胞悬浮培养物上培养的病毒生产的口蹄疫疫苗必须满足的标准。