用于直接检测尿液样本中BK病毒的斑点酶联免疫吸附测定法。
Dot ELISA for direct detection of BK virus in urine samples.
作者信息
Marrero M, Pascale F, Alvarez M, De Saint Maur G, Rosseau E, Nicolas J C, Garbarg-Chenon A, Bricout F
机构信息
Department of Virology, Institute of Tropical Medicine, Pedro Kouri, Havana City, Cuba.
出版信息
Acta Virol. 1990 Dec;34(6):563-7.
The dot ELISA technique was applied for direct detection of BK virus in clinical urine samples. The assay was performed on nitrocellulose paper dotted with the polyethylene glycol precipitated urine samples free of cellular debris. BK virus was detected with an anti-BK virus monoclonal antibody, and the complex was visualized by immunoperoxidase staining. Positive reaction appeared as well-defined dark blue spots. Of the 110 urine samples examined, 31 were positive in the dot ELISA and 79 proved negative. Comparing with the IIF results, the dot ELISA had a 88.46% of sensibility and 90.4% of specificity, and the results agreed completely in 99 samples. The simple dot ELISA technique can be recommended for detection of BK virus excretion in routinary diagnostic.
采用斑点酶联免疫吸附测定（dot ELISA）技术直接检测临床尿液样本中的BK病毒。检测在硝酸纤维素纸上进行，纸上点样的是经聚乙二醇沉淀且无细胞碎片的尿液样本。用抗BK病毒单克隆抗体检测BK病毒，通过免疫过氧化物酶染色使复合物显色。阳性反应呈现为界限清晰的深蓝色斑点。在检测的110份尿液样本中，斑点酶联免疫吸附测定有31份呈阳性，79份为阴性。与间接免疫荧光法（IIF）结果相比，斑点酶联免疫吸附测定的灵敏度为88.46%，特异性为90.4%，99份样本的结果完全一致。简单的斑点酶联免疫吸附测定技术可推荐用于常规诊断中BK病毒排泄的检测。
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